R659S mutation of γPKC is susceptible to cell death: Implication of this mutation/polymorphism in the pathogenesis of retinitis pigmentosa

It has been reported that mutations of γPKC cause hereditary spinocerebellar atrophy type 14 (SCA14). Our recent study has revealed that the SCA14 mutant γPKC is susceptible to aggregation and causes cell death. Among mutations/polymorphisms of γPKC, the R659S mutation was firstly segregated from families with hereditary retinitis pigmentosa type 11 (RP11). Although more reliable etiological mutations of RP11 were subsequently discovered in a human homologue of yeast pre-mRNA splicing gene (PRP31), the role of this R659S missense change in the pathogenicity of RP11 is still controversial. In this study, we overexpressed R659S γPKC in CHO cells and characterized the properties of this mutant protein. We found that R659S γPKC more prominently induced cell death than did wild-type. This mutant γPKC had higher basal activity than wild-type, however, no difference was found in the extent of aggregation and insolubility to detergent between R659S mutant and wild-type. These results suggest that the R659S mutation is susceptible to neuronal death and is involved in the pathogenesis of neurodegenerative diseases, including RP11.