کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2202331 | 1100097 | 2006 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Firing of 5-HT neurones in the dorsal and median raphe nucleus in vitro shows differential α1-adrenoceptor and 5-HT1A receptor modulation Firing of 5-HT neurones in the dorsal and median raphe nucleus in vitro shows differential α1-adrenoceptor and 5-HT1A receptor modulation](/preview/png/2202331.png)
The median raphe nucleus and dorsal raphe nucleus together are the major source of ascending 5-HT projections. Here, using in vitro extracellular single unit electrophysiology we examined the responses of individual neurones in the rat median raphe nucleus and dorsal raphe nucleus to α1-adrenoceptor and 5-HT1A receptor activation and made comparisons between the two nuclei. In the presence of the α1-adrenoceptor agonist phenylephrine (1 μM) all spontaneously active neurones recorded in the median and dorsal raphe nuclei fired slowly (<5 Hz) and regularly. Most were inhibited by 5-HT (10–50 μM), although a few were excited by 5-HT. 5-HT-induced inhibition was attenuated by the 5-HT1A receptor antagonist, WAY100635 (100 nM). Compared to those in the dorsal raphe nucleus, the neurones in the median raphe nucleus which were inhibited by 5-HT had: (1) lower basal firing rates in the continuous presence of phenylephrine (1 μM), (2) smaller excitatory responses to higher concentrations of phenylephrine (3–10 μM), (3) smaller excitatory responses to brief application of norepinephrine (10–100 μM) and (4) smaller inhibitory responses to 5-HT (10–50 μM). The lower sensitivity of median raphe neurones to α1-adrenoceptor excitation and 5-HT1A receptor inhibition will have consequences for 5-HT neurotransmission in forebrain regions innervated by the two nuclei.
Journal: Neurochemistry International - Volume 48, Issue 2, January 2006, Pages 100–107