کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2202432 1551337 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fas/FasL and perforin–granzyme pathways mediated T cell cytotoxic responses in infectious bursal disease virus infected chickens
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Fas/FasL and perforin–granzyme pathways mediated T cell cytotoxic responses in infectious bursal disease virus infected chickens
چکیده انگلیسی

Infectious bursal disease (IBD) is a highly contagious disease of chickens which leads to immunosuppression. In our previous study it was demonstrated that, possibly, CD4+ and CD8+ T cells may employ perforin and granzyme-A pathway for the clearance of IBDV-infected bursal cells. In this study, we evaluated the cytotoxic T cell responses involving two independently functioning but complementary mechanisms: Fas–Fas ligand and perforin–granzyme pathways in IBDV-infected chickens. As demonstrated previously, infection of chickens with IBDV was accompanied by influx of CD8+ T cells in the bursa and spleen. There was an upregulation in the gene expression of cytolytic molecules: Fas and Fas ligand (FasL), perforin (PFN) and granzyme-A (Gzm-A) in bursal and in the splenic tissues of IBDV inoculated chickens. Additionally, for the first time, we detected Fas, Fas ligand, Caspase-3 and PFN producing CD8+ T cells in the bursa and spleen of IBDV-infected chickens. The infiltration and activation of CD8+ T cells was substantiated by the detection of Th1 cytokine, IFN-γ. These data suggest that T cells may be involved in the clearance of virus from the target organ bursa and peripheral tissues such as spleen. The findings of these studies provide new insights into the pathogenesis of IBD and provide mechanistic evidence that the cytotoxic T cells may act through both Fas–FasL and perforin–granzyme pathways in mediating the clearance of virus-infected cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Results in Immunology - Volume 2, 2012, Pages 112–119
نویسندگان
, , , ,