Establishment of recipient model for spermatogonial stem cells transplantation in Kunming mice

• The effective recipient model for SSCs transplantation in Kunming mice was established after different doses busulfan treatment.
• The optimal dose of busulfan was 20 mg/kg and the most appropriate time for transplantation was 5–7 wk after busulfan treatment.
• Donor-derived cloned fragments in the testis of recipient mice and offspring were produced.

The objective of this study was to establish a recipient model for spermatogonial stem cells (SSCs) transplantation in the Kunming mice after different doses busulfan treatment. The results showed that the most optimal dose of busulfan was 20 mg/kg and the most appropriate time for transplantation was 5–7 wk after busulfan treatment. Then, the cloned fragments existed in the testis of recipient mice after 20 mg/kg busulfan treatment and the offspring with enhanced green fluorescent protein (EGFP) were produced by the transplanting SSCs. Hence, we established the effective recipient model for donor-derived SSCs transplantation in Kunming mice.

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