Roles of endosomal trafficking in neurite outgrowth and guidance

Membrane trafficking and cargo delivery are essential for axonal and dendritic growth and guidance. Neurons have numerous diverse post-Golgi vesicles and recent advances have clarified their identity and regulation. Combinatorial approaches using in vivo imaging of ‘intracellular cargo address labels’ and functional perturbation have provided insight into these processes. In particular, the UNC-51 kinase regulates the trafficking of early endosomes and their axon guidance molecular cargos in several types of neurons in multiple organisms. Vesicular compartments bearing features of recycling endosomes, late endosomes or lysosomes also contribute to membrane addition and protein trafficking during neurite outgrowth and extension. New work shows that ubiquitylation of cargos and Rab effectors further specifies the trafficking routes of post-Golgi vesicles. These findings have begun to provide a more detailed view of the molecular mechanisms involved in neurite outgrowth and guidance. Additionally, high-resolution light microscopy imaging promises greater temporal and spatial understanding of vesicular exchange and maturation in neurons in the near future.