کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
228135 | 464835 | 2012 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Multiple sequence alignment-inspired mutagenesis of marine epoxide hydrolase of Mugil cephalus for enhancing enantioselective hydrolytic activity Multiple sequence alignment-inspired mutagenesis of marine epoxide hydrolase of Mugil cephalus for enhancing enantioselective hydrolytic activity](/preview/png/228135.png)
The marine fish microsomal epoxide hydrolase (mEH) of Mugil cephalus was engineered to enhance the enantioselective hydrolytic activity by multiple sequence alignment-inspired mutagenesis. The amino acid sequences of Aspergillus niger, Rhodotorula glutinis, zebra fish and human mEH were aligned and analyzed for identifying target amino acids. Single-point mutants (Q170K, E186K, E378D) and double-point mutants (E378D-Q170K, E378D-Y348F, E378D-Y348H) were developed and their hydrolytic activities were compared. The double-point mutant, E378D-Q170K, exhibited an enhanced hydrolytic activity by 4.6-fold, compared to the wild-type M. cephalus mEH. Enantiopure (S)-styrene oxide could be readily prepared with high enantiopurity more than 99%ee by using the double-point mutant.
Journal: Journal of Industrial and Engineering Chemistry - Volume 18, Issue 1, 25 January 2012, Pages 72–76