کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
228881 | 464852 | 2010 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Kinetics of Salmonella typhimurium binding with antibody by immunoassays using a surface plasmon resonance biosensor
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
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چکیده انگلیسی
This study examined the kinetics of Salmonella typhimurium binding to an antibody using a direct assay and sandwich assay with a surface plasmon resonance (SPR) biosensor. A kinetic model was proposed based on curve-fitting of the experimental SPR sensorgrams using an integral rate equation. The SPR sensitivity in the sandwich assay was significantly higher than that in the direct assay. The association rate constants and affinities of the cell in the sandwich assay were double those in the direct assay. The enhanced binding attraction between the cell and detection antibody in the sandwich assay improved the lower limit of detection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Industrial and Engineering Chemistry - Volume 16, Issue 1, 25 January 2010, Pages 115–118
Journal: Journal of Industrial and Engineering Chemistry - Volume 16, Issue 1, 25 January 2010, Pages 115–118
نویسندگان
Kyong-Hwan Chung, Hyun-Soo Hwang, Ki-Young Lee,