Applicability of sub- and supercritical water hydrolysis of woody biomass to produce monomeric sugars for cellulosic bioethanol fermentation

In this study two woody biomasses, poplar and pitch pine wood, were treated with sub- and supercritical water (SCW) at temperature of 325–425 °C, at pressure of 220 ± 10 atm and residence time of 60 s, respectively, to develop a time saving and efficient conversion process for the production of fermentable sugars from woody biomasses using supercritical water system. Cellulose/hemicellulose was easily hydrolyzed during SCW treatment into monomeric sugars with the total yield of 7.3% and 8.2% based on the oven dried weight of poplar and pitch pine, respectively. Total yield of the monomeric sugars was increased about threefolds to 23.0% and 25.1% in the presence of 0.05% of hydrochloric acid. Model experiment confirmed that glucose and xylose were readily converted into low molecular weight compounds during SCW hydrolysis. According to GC/MS analysis main compounds converted from glucose and xylose by SCW were identified to 5-hydroxymethyl furfural and 4-oxo-5-methoxy-2-penten-5-olide, respectively.