Decolourization potential of white-rot fungus Phanerochaete chrysosporium on synthetic dye bath effluent containing Amido black 10B

Synthetic azo dyes are extensively used in textile industry and are not easily degraded into the environment due to their complex structure. Due to the low degree of fixation of these dyes to fabrics, more than 10–15% of the dye does not bind to fabrics during colour processing and release into water bodies as effluent cause serious environmental pollution. White-rot fungus is found to be capable of degrading lignin which has a complex structure similar to azo dyes. In this study, the decolourization potential of white-rot fungus Phanerochaete chrysosporium, which is capable of decolourizing synthetic dye bath effluent, was investigated. Maximum decolourization of 98% was achieved on the third day under normal conditions. The rate of decolourization carried out at different concentrations revealed that the increase in dye effluent concentration suppresses the percentage decolourization. The optimized amounts of nutrients were found to be 0.5%, 0.1% and 0.5% of glucose, manganese sulphate and ammonium salts, respectively. The addition of inducers such as starch and lignin increased enzyme production and the rate of decolourization.

► Biodecolourization studies carried out for synthetic dye bath effluent containing Amido black 10B using Phanerochaete chrysosporium.
► This fungus has shown maximum removal of 98% an the third day.
► The addition of carbon and nitrogen sources (glucose, manganese and ammonium salts) was investigated.
► The effect of external aeration and after addition has been studied for dye bath effluent.
► The influence of Co substrates such as lignin and starch has also been studied.