کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
231224 | 1427423 | 2011 | 7 صفحه PDF | دانلود رایگان |
This study employed column elution fractionation coupled with supercritical anti-solvent recrystallization to isolate the purest zeaxanthin dipalmitates (ZP), one of the eye-protecting compounds in the Lycium barbarum fruits. The amount of ZP increased to 983 mg/g in liquid anti-solvent precipitates with 29.1% recovery and increased to 929 mg/g in supercritical anti-solvent precipitates with 59.6% recovery, respectively from the ultrasonic acetone extract. Experimental data showed that supercritical anti-solvent recrystallization is feasible in producing platelet type precipitates within a few minutes. These ZP compounds exhibit anti-oxidative abilities by proliferating human adult retinal pigment epithelial cells (from 22% to 31%); though it was a meager increase when compared to those of algal 95% zeaxanthin (from 43% to 94%).
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► Supercritical carbon dioxide anti-solvent recrystallization was employed in rapidly producing micro-sized zeaxanthin dipalmitates (ZP) as eye-sight protecting health supplements from Lycium barbarum fruits.
► De-glycosides pre-treatment was necessary in obtaining the purest ZP compound.
► Anti-oxidation ability of ARPE-19 cells was successfully examined for these ZP compounds.
Journal: The Journal of Supercritical Fluids - Volume 57, Issue 2, June 2011, Pages 155–161