کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2395205 1101559 2014 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Application of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry in Identification of Stallion Semen Bacterial Contamination
ترجمه فارسی عنوان
استفاده از اسپکترومتر جرمی یونیزاسیون لیزر جذب ماتریس در شناسایی علف های هرز باکتریایی علف های هرز
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is one of the cutting-edge methods currently applied in medical bacteriologic diagnostics. The aim of the study was to prove the possibility of applying MALDI-TOF MS to identify bacterial contamination in the ejaculate of stud stallions, which may cause infections to reproductive organs of mares following artificial insemination with cooled semen. A partial aim was to determine changes in the total count of microorganisms in long-term storage of ejaculate after its treatment with gentamicin and also without antimicrobial medication. Aerobic cultivation on Columbia agar was used to examine 26 semen samples from 13 horses; 31 different species of bacteria were isolated, which were identified by MALDI-TOF MS. The most frequently detected species came from Aerococcaceae, Staphylococcaceae, and Micrococcaceae families. The results of our work confirm that MALDI-TOF MS is a quick alternative method for identifying bacterial species that may contaminate stallion semen.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Equine Veterinary Science - Volume 34, Issue 6, June 2014, Pages 833–836
نویسندگان
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