کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2395826 | 1101588 | 2010 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Use of Direct Thaw Insemination to Establish Pregnancies with Frozen-Thawed Semen from a Standard Jack
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
Pregnancy rates reported after artificial insemination with frozen-thawed jack spermatozoa have been relatively low compared with those attained in other species. Cholesterol is known to influence post-thaw fertility of both jack and stallion semen, and altering the amount of cholesterol in the freezing extender may help improve the fertility of frozen-thawed jack semen samples. In this study, we report clinical work that was performed using semen samples collected from a single jack. Samples were extended in EZ Mixin OF and then slowly cooled to 5°C. Extended semen samples were centrifuged at 400 à g for 10 minutes and the supernatant was discarded. Spermatozoa were resuspended in freezing medium to a final concentration of 400 à 106 cells/mL and were later frozen in liquid nitrogen vapor. Freezing extender treatments containing 2% ethylene glycol included the following: (1) 20% egg yolk (EY), (2) 5% EY, and (3) 20% EY + 60 mM hydroxypropyl-β-cyclodextrin (β-CD). For this study, a total of 28 mares aged 2 to 18 years was used over five breeding seasons (82 total cycles). Mares were administered human chorionic gonadotropin to induce ovulation when the dominant follicle was â¥35 mm in diameter. They were inseminated within 6 hours before ovulation and again within 6 hours after ovulation. Pregnancy rates obtained were as follows: (1) 6.25% (one of 15 matings) for 20% EY, (2) 46.5% (20 of 43 matings) for 5% EY, and (3) 58.5% (14 of 24 matings) for 20% EY + 60 mM β-CD. These data suggest that binding of cholesterol with β-CD enhances post-thaw fertility of jack semen samples. We conclude that acceptable pregnancy rates could be achieved with frozen-thawed jack semen samples cryopreserved in 5% EY or 20% EY + 60 mM β-CD using direct post-thaw insemination.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Equine Veterinary Science - Volume 30, Issue 11, November 2010, Pages 651-656
Journal: Journal of Equine Veterinary Science - Volume 30, Issue 11, November 2010, Pages 651-656
نویسندگان
Rebecca J. DVM, MS, PhD, Lawrence E. DVM, PhD, Curtis R. PhD,