Synthesis of Hydroxyapatite from Rice Fields Snail Shell (Bellamya javanica) through Wet Method and Pore Modification Using Chitosan

Hydroxyapatite (HAp) was synthesized using calcium originated from rice fields snail shell, that was reacted with phosphate obtained from diammonium hydrogen phosphate. The resulted pore was modified using chitosan. The HAp was prepared using wet method. Chitosan was used as porogen due to its bioactivity and biocompatibility properties. Sonication time in the synthesis was varied in 2, 4, and 6hours. The longer the synthesis time, the higher the purity of the HAp. Pore modification was applied to HAp with the highest purity. Pore modifications were conducted by adding 4% and 6% chitosan. Scanning electron microscope identification showed no significant difference in pore sizes using 4% and 6% chitosan. Fourier transform infrared spectrum of HAp with 6% chitosan showed almost similar functional groups with that of the unporogened HAp. In vitro test of the porogened HAp revealed its bioactivity.