کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
239706 466199 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel immunoassay using platinum nanoparticles, silver enhancement and a flatbed scanner
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
A novel immunoassay using platinum nanoparticles, silver enhancement and a flatbed scanner
چکیده انگلیسی

This study reports a potentially rapid and convenient immunoassay using antibody-platinum nanoparticle (Ab-PtNPs) conjugates as a reporter molecule and a flatbed scanner for detecting immuno-reaction and measuring of the immuno-reaction signal. This study was based on the sandwich immunoassay (three-layer format) which contained a primary antibody, test antigens and a secondary antibody. The results showed that the silver precipitation phenomenon was catalyzed by Ab-PtNPs conjugates. The changing color of reaction could easily be observed by naked eye or scanner. The silver enhancement reaction, a signal amplification method in which silver ions are reduced to silver metal, is introduced to magnify the detection signal. The relationship between sample concentration and detection signal was discussed. And the detection limit (sandwich assay) for the sample antigen was 10-1 ng/mL. Using a flatbed scanner, Ab-PtNPs conjugates and a silver enhancement reaction, a new immunoassay is constructed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Procedia Chemistry - Volume 1, Issue 1, September 2009, Pages 256-260