کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
239765 | 466228 | 2012 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Development of a Disposable Gold Electrodes-Based Sensor for Electrochemical Measurements of cDNA Hybridization Development of a Disposable Gold Electrodes-Based Sensor for Electrochemical Measurements of cDNA Hybridization](/preview/png/239765.png)
This work deals with the development of a disposable electrochemical biosensor for the specific detection of short DNA sequences. The sensor is an amperometric transducer with three planar electrodes, comprising a working, a counter and a pseudo-reference electrode, all made of a gold layer over a polycarbonate substrate. For the development of the genosensor, the working electrode was modified using thiol-tethered 33-mer DNA probe by chemisorptions, in a concentration range from 0.1 μM to 5 μM. Immobilization of ssDNA on gold surface was monitored with electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) in Fe(CN)64−/13− and Ruthenium(II)/(III) solutions. The time dependence of ssDNA probe immobilization was also studied. The hybridization detection is then compared with EIS and DPV measurements.
Journal: Procedia Chemistry - Volume 6, 2012, Pages 36-45