An Electrochemical Biosensor for the Determination of Lactic Acid in Expiration

We have successfully developed an electrochemical biosensor which could detect low level concentration of misty lactic-acid (LA) directly. In our method, enzyme lactate oxidase (LOD) was immobilized within mesoporous silica (FSM8.0) which was formed on screen-printed Prussian Blue (PB)-modified working electrode and the top of that was covered with hydrophilic porous membrane. The resulting biosensor showed an excellent sensitivity (150nM-1.1 mM in solution) and can detect misty LA directly. This result indicates that our method provides a high-performance biosensor to determine LA levels in exhaled breath and that our approach is ideal for various medical applications and might improve the detection or management of disease.