Hydrolysis of Corncob Xylan using β-xylosidase GbtXyl43B from Geobacillus Thermoleovorans IT-08 Containing Carbohydrate Binding Module (CBM)

Corncob is mainly compose of lignocellulose rich in xylan. Corncob collected from Tanjung Anom village, East Java, was extracted by alkaline method. Corncob was used as substrate for β-xylosidase GbtXyl43B and oat spelt xylan was used as standard for xylan substrate. GbtXyl43B has molecular mass of 72 kDa and is composed of Catalytic Module (CM) and Carbohydrate Binding Module (CBM). GbtXyl43B was purified from culture supernatant using Ni-NTA affinity chromatography. The enzyme was purified 26.785 fold over the crude extract of soluble proteins with a specific activity of 14.9641 Unit mg-1. Enzymatic hydrolysis product of corncob and oat spelt xylan was analyzed by HPLC, producing xylose, arabinose and xylooligosaccharide.