Biotransformation of Eugenol to Dehydroeugenol Catalyzed by Brassica juncea Peroxidase and its Cytotoxicity Activities

A crude peroxidase biocatalyst extracted from sawi hijau (Brassica juncea) was used to produce dimerization phenolic synthesized from eugenol. The result from MS and NMR analysis showed that the reaction between eugenol substrate and H2O2 with crude extract enzyme produced a new chemical compound. The chemical structure of new compound showed coupled result in ortho-ortho position with C-C coupled derivative, dehydrodieugenol. It was obtained as brownish white crystal needles with yield 10%. This compound displayed higher cytotoxicity effect on T47D breast cancer cell compared to the eugenol monomer.