Apoptosis in Mice Liver Cells Caused by Formalin–containing Food: Normalization of HSP70 Overexpression by Chlorophyllin

Repeated exposure to formalin–containing foodstuff results in mice to overexpressing HSP70 (Heat Shock Protein, 70 kDa) and induction of apoptosis. This study tried to assess the efficacy of supplementation of chlorophyllin in inhibiting this induced apoptosis. In this study, a 4 x 4 factorial experiment was conducted with treatment type and duration of exposure as independent variables. Expression of HSP70 was determined by immunohistochemistry (avidin–biotin sandwich assay). The number of cells expressing HSP70 was statistically analyzed using two–way ANOVA, followed by Duncan's Multiple Range Test (DMRT, α = 0.01). From the results, it can be concluded that chlorophyllin supplementation is able to inhibit the number of cells undergoing induced apoptosis due to repeated exposure of formalin–containing fish by normalizing the overexpressing of HSP70.