The Level of Glutamic Acid in the Semen of Male White Rat (Ratus Norwegicus) after Being Treated with Tannin of Pluchea Indica

This current research aims at proving that the treatment of Pluchea indica's tannin could affect the level of glutamic acid in the semen of male white rat. Experimental method was employed, under the following details: control group (without being treated) and experiment group with the treatment of Pluchea indica's tannin. The observation was conducted based upon the treatment; the white rats were dissected, and vas deferens is taken to get the semen of the white rat. The amino acid of the semen was then analyzed by utilizing high-performance liquid chromatography. The data are analyzed by using ANOVA. The results show that there is an effect after the treatment of Pluchea indica's tannin on the level of glutamic acid in the semen of male white rat (p < 0.05). The male white rats in the control group had averagely 1 547.48 mg · (100 g)–1 glutamic acid level; while the male white rats in the experiment group (after the treatment of Pluchea indica's tannin) showed the decrease on glutamic acid level (1 494.43 mg · (100 g)–1 to 1 341.40 mg · (100 g)–1). The high and low level of glutamic acid determined the quality of spermatozoa.