In Vitro Selection of Jatropha Curcas Linn. Hybrids Using Polyethylene Glycol to Obtain Drought Tolerance Character

This research aims to repair the character of drought tolerance at F1 hybrids of Jatropha curcas Linn. by in vitro selection using polyethylene glycol. The seeds of J. curcas genotypes were sterilized before germinated on a sterile sand medium. The zygotic embryos then grown in MS media + 0.5 mg L–1 BAP + 0.4 mg L–1 TDZ. The lethal dossage value was reached 73.57% from treatment of 15% PEG, and 20% of PEG treatment was reached 91.43%. In selection media, the percentage of death explants ranged from 76.00% to 93.50%. The callus that obtained, then propagated and developed into regeneration media and expected could be developed completely plantlets which would have drought tolerance.