کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
240434 | 466268 | 2014 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Characterization of Peroxidase Enzyme from Water Spinach (Ipomoea Aquatica Forssk.) Fraction Characterization of Peroxidase Enzyme from Water Spinach (Ipomoea Aquatica Forssk.) Fraction](/preview/png/240434.png)
Peroxide enzymes are widely used as a reagent in many clinical diagnosis of analysis methods such as ELISA, immunoblotting, and immunochemistry. Water spinach (Ipomea aquatica Forssk) is a species of plant available abundantly in Indonesia, hence it is a potential source of peroxidase. In this study, the crude extract of water spinach leaves was known to contain peroxidase enzyme which was active toward hydrogen peroxide. A fraction of extract yielded from differential fractionation using ammonium sulfate at concentration 40-65% (F4) was known to be the most active. Characterization of specific activity of peroxidase of F4 for optimum pH and temperature was at pH 5 and 60 °C. The Michaelis-Menten constant (Km) was 0,27 mg/mL and enzyme's catalytic rate (Vmax) was 2,16 mg/mL/min.
Journal: Procedia Chemistry - Volume 13, 2014, Pages 170-176