Characterization of Peroxidase Enzyme from Water Spinach (Ipomoea Aquatica Forssk.) Fraction

Peroxide enzymes are widely used as a reagent in many clinical diagnosis of analysis methods such as ELISA, immunoblotting, and immunochemistry. Water spinach (Ipomea aquatica Forssk) is a species of plant available abundantly in Indonesia, hence it is a potential source of peroxidase. In this study, the crude extract of water spinach leaves was known to contain peroxidase enzyme which was active toward hydrogen peroxide. A fraction of extract yielded from differential fractionation using ammonium sulfate at concentration 40-65% (F4) was known to be the most active. Characterization of specific activity of peroxidase of F4 for optimum pH and temperature was at pH 5 and 60 °C. The Michaelis-Menten constant (Km) was 0,27 mg/mL and enzyme's catalytic rate (Vmax) was 2,16 mg/mL/min.