Enantioseparations of aromatic carboxylic acid by capillary electrophoresis using eremomycin as a chiral selector in a chitosan-modified capillary

The adsorption of macrocyclic antibiotic eremomycin on the capillary wall has been studied and concentration of EOF reversion has been determined. The combination of coupled chitosan coating and macrocyclic antibiotic as run buffer additive has been proposed for chiral separation. The advantages (time analysis and efficiency) of coupled chitosan-coated capillary over fused-silica capillary have been shown. The effect of experimental parameters, influencing the enantioseparation, has been investigated. Carboxylic acids bearing α-C*-asymmetric carbon atom (ibuprofen, indoprofen, ketoprofen, fenoprofen, flurbiprofen, mandelic, 3-phenylbutiric, 2-phenoxypropionic, and α-methoxyphenylacetic acids) have been enantioseparated with the separation selectivity being more than 1.04. The analysis time was less than 9 minutes