کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
240593 | 466271 | 2010 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Coupling of capillary electrophoresis with reaction detection for the on-line evaluation of radical scavenging activity of analytes Coupling of capillary electrophoresis with reaction detection for the on-line evaluation of radical scavenging activity of analytes](/preview/png/240593.png)
The main task of this work was to create a rapid, simple and less material and time consuming method involving capillary electrophoretic separation in order to separate analytes and evaluate antioxidant activity within a single analytical run. Several interfaces were developed and used to couple CE to the reaction detector. The method developed enables simultaneous electrophoretic separation and evaluation of antioxidant activity of each separated compound in the mixture. The analysis was performed using DPPH (2,2-diphenyl-1-picrylhydrazyl) as synthetic radical reagent. The on-line capillary electrophoresis-reaction (antioxidant activity) detection method can be used for a rapid evaluation of individual antioxidants in complex mixtures, particularly extracts of natural products. Possibility to evaluate radical scavenging activity of extracts of natural products components is demonstrated on an example of aqueous propolis extract. Four phenolic acids where separated and their radical scavenging activity was on-line evaluated.
Journal: Procedia Chemistry - Volume 2, Issue 1, January 2010, Pages 54-58