Are conspecific and heterospecific opponents assessed similarly? A test in two species of territorial damselfish (Pomacentridae)

• The Stegastes diencaeous and S. adustus compete for territories on coral reefs.
• We examined whether species use different assessment tactics for different species.
• Using an opponent′s body size, both species differ in their assessment tactics.
• However, both species treated the other species as if were a conspecific intruder.

Aggression is often a crucial component to interference interspecific competition and yet there are few studies that examine fight behavior when the opponents are different species. To examine conspecific and heterospecific aggression, we used two species of Caribbean damselfish, the dusky (Stegastes adustus) and the longfin (S. diencaeous) with each one serving as the heterospecific opponent to the other species. Our study was confined to whether or not each species measures the body length of the other species as if it were a conspecific intruder. Body length plays an important role in fight outcome in many species of fish and we presumed that both the dusky and the longfin would use it when assessing opponents. Both the dusky and the longfin damselfish were then presented with two individuals that were either greatly different or minimally different in size. When presented with individuals that differed greatly in size, both species spent more time attacking the larger individual, irrespective of species. However, when the size difference was small, the focal dusky and the longfin responded differently; the dusky showed no preference in either conspecific or heterospecific pairs while the longfin continued to show a bias toward the slightly larger individual. Thus, while we were surprised by the species differences, both the dusky and the longfin were internally consistent in how they treated conspecific and heterospecific opponents.