کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
2428844 1553577 2016 4 صفحه PDF سفارش دهید دانلود رایگان
عنوان انگلیسی مقاله ISI
Differential expression of microRNAs in shrimp Marsupenaeus japonicus in response to Vibrio alginolyticus infection
ترجمه فارسی عنوان
بیان متمایز میکرو RNA ها در میگو Marsupenaeus japonicus در پاسخ به عفونت Vibrio alginolyticus
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کلمات کلیدی
microRNA؛ Marsupenaeus japonicus؛ alginolyticus ارتعاشی؛ توالی RNA کوچک؛ تجزیه و تحلیل GO؛ RespemiRNA ایمنی، microRNAs؛ pri-miRNA، primary-miRNA؛ مناطق 3'-UTR، 3'-untranslated؛ WSSV، ویروس سندرم نقطه سفید ؛ GO، Gene Onology؛ RIN، RNA integrity nu
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
چکیده انگلیسی


• The small RNA sequencing of Vibro alginolyticus-challenged shrimp was firstly performed.
• 55 host miRNAs were identified that differentially expressed in response to V. alginolyticus infection.
• The differentially expressed miRNAs contribute to the innate immunity like phenoloxidase, apoptosis and phagocytosis.

Till date numerous microRNAs (miRNAs) have been discovered from various organisms, including mammals, plants, insects, nematodes and viruses. They are known to have antiviral functions in crustaceans such as shrimp Marsupenaeus japonicas. However, little is known about the role of miRNAs against bacterial infection in this shrimp caused by Vibrio alginolyticus. We performed small RNA sequencing to characterize the differentially expressed microRNAs in V. alginolyticus challenged shrimp, in comparison to that in control uninfected shrimp, at 24 h and 48 h. In total, 55 host miRNAs were differentially expressed in response to the infection and most of these were downregulated at both the time-points. TargetScan and miRanda algorithms showed that the target genes of these down-regulated miRNAs were related to innate immune functions such as production of phenoloxidase enzyme, apoptosis and phagocytosis. Further, gene ontology analysis revealed that many immune signaling pathways were mediated by these miRNAs. This study is one of the earliest attempts at characterizing shrimp miRNAs that respond to V. alginolyticus infection, and will help unravel the miRNA pathways involved in antibacterial action in shrimp.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 55, February 2016, Pages 76–79
نویسندگان
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