|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|2430803||1553619||2016||5 صفحه PDF||سفارش دهید||دانلود رایگان|
• Dietary Galactooligosaccharide (GOS) improved mucosal immune response in goldfish.
• GOS fed Goldfish showed elevated systemic immune response.
• Dietary GOS down-regulated appetite related gene (ghrelin) expression in goldfish.
• Immune related genes were up-regulated in GOS fed goldfish.
The present study investigates the effects of supplementation of goldfish (Carassius auratus gibelio) diet with galactooligosaccharide (GOS) on serum immune response, mucosal immune parameters as well as appetite-related (Ghrelin) and immune-related (TNF-1α and TNF-2α) genes expression. One hundred and eighty fish with an average weight of 4.88 ± 0.28 g were stocked in twelve 500-L fiberglass tank assigned to four treatments repeated in triplicates. Fish were fed on experimental diets contain 0.5, 1 and 2% GOS for 6 weeks. Supplementation of diet with GOS had no remarkable effect on goldfish growth performance (P > 0.05). Evaluation of serum innate immune parameters revealed that supplementation of diet with GOS significantly elevated total protein, Albumin, Globulins, Lysozyme and Alkaline phosphatase activity as well as agglutination compared to control group in a dose dependent manner (P < 0.0.5). Also, Fish fed 2% GOS supplemented diet showed increased skin mucus immune response (total protein and lysozyme activity) compared other groups (P < 0.0.5); except in case of ALP activity. Molecular studies on appetite (ghrelin) and inflammatory cytokine (TNF-1α and TNF-2α) genes expression revealed remarkably decrease and increase, respectively in GOS fed fish (P < 0.0.5). These results showed immunomodulatory effects of dietary GOS on serum and skin mucus response as well as expression of inflammatory cytokines in goldfish, though this supplement decreased appetite gene expression and had no effect on growth performance.
Journal: Fish & Shellfish Immunology - Volume 55, August 2016, Pages 479–483