کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2433876 1553669 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of kinetic parameters for casein hydrolysis by chymotrypsin using two ranges of substrate concentration
ترجمه فارسی عنوان
تعیین پارامترهای جنبشی برای هیدرولیز کازئین توسط کیموتریپسین با استفاده از دو محدوده غلظت سوبسترا
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی

Hydrolysis of casein by chymotrypsin was performed at low and high substrate concentrations. Hydrolysis kinetics were analysed in the framework of a two-step model, with consecutive demasking and hydrolysis steps. This model took into consideration the limited accessibility of peptide bonds for the enzyme (masking effect). Dependence of the logarithm of the hydrolysis rate via degree of hydrolysis was non-linear at concentrations of 0.04 and 10 g L−1. In the intermediate range of substrate concentrations (0.07–0.72), this dependence was found to be linear, corresponding to the exponential model of proteolysis. Taking into consideration the multiplicity of peptide bonds, coupled to peptide bond demasking, allowed determination of two important parameters: the ratio of the rate constants of demasking and hydrolysis (kd/kh), and the fraction of initially masked bonds (m). An analytical procedure was suggested for the calculation of the apparent Michaelis constants at various degrees of hydrolysis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Dairy Journal - Volume 61, October 2016, Pages 76–84
نویسندگان
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