کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2433967 1553675 2016 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of a quantitative immuno-affinity test column assay for on-site screening of clindamycin residues in milk
ترجمه فارسی عنوان
بررسی تست ستون آزمون ایمنی کمیاب برای بررسی غربالگری بقایای کلیندامایسین در شیر
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی

A sensitive and specific monoclonal antibody against clindamycin (CLIN) was produced and used to develop an immuno-affinity test column (IATC) assay for on-site screening of CLIN residues in milk. The qualitative limit of detection of the IATC assay, estimated as 1.0 μg L−1 by visual detection, was sufficient to measure maximum residue levels for lincosamide antibiotics in milk. The quantitative IATC assay resulted in a lower detection limit (0.11 μg L−1) by evaluating the colour intensity of the test layers, which was approximately 9 times greater sensitivity than visual detection. During the spike and recovery test, the average recoveries ranged from 76% to 114% at different spiked levels, and the intra-/interday coefficients of variation were in the range 9.6–16.7%. The detection time was shortened to 20 min, whereas the sensitivity was comparable with a traditional ELISA. The IATC assay shows promise for on-site screening of CLIN residues in milk.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Dairy Journal - Volume 55, April 2016, Pages 59–63
نویسندگان
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