Effects of treating rice straw with urea or urea and calcium hydroxide upon intake, digestibility, rumen fermentation and milk yield of dairy cows

Three, multiparous Holstein crossbred dairy cows with initial body weight of 385 ± 19 kg were randomly allocated to 3 treatments of rice straw (T1 = untreated rice straw; T2 = 5.5% urea-treated rice straw (5 g urea in 100 ml water to 100 g air-dry (91% DM) straw); T3 = 2.2% urea + 2.2% calcium hydroxide treated rice straw (2.0 g urea and 2.0 g Ca(OH)2 in 100 ml to 100 g air-dry (91% DM) straw) according to a 3 × 3 Latin square design. Each period lasted for 21 days in which feed, feces and rumen fluid were collected during the last 7 days for chemical analyses. The findings revealed significant improvements in dry matter intake and digestibility by using 5.5% urea-treated rice straw and 2.2% urea + 2.2% calcium hydroxide treated rice straw. Ruminal pH and NH3-N were found higher (P < 0.05) as compared with urea-treated rice straw fed group, while blood urea nitrogen and milk urea nitrogen were in normal ranges. Volatile fatty acid concentrations especially those of acetic acid were decreased (P < 0.05) and those of propionic acid were increased (P < 0.05), thus acetic acid:propionic acid was subsequently lowered (P < 0.05) in cows fed with 5.5% urea-treated rice straw and 2.2% urea + 2.2% calcium hydroxide treated rice straw, respectively. Moreover, ruminal viable and cellulolytic bacterial counts were enhanced by urea and calcium hydroxide treatments. Milk protein and fat concentrations were additionally increased by respective treatments while 3.5% fat-corrected milk was highest; ranking from 2.2% urea + 2.2% calcium hydroxide treated rice straw, 5.5% urea-treated rice straw and urea-treated rice straw fed groups. Based on this study, implications could be made that using 2.2% urea + 2.2% calcium hydroxide treated rice straw for straw treatment could be an alternative treatment to 5.5% urea treatment with regards to its effectiveness and treatment cost for lactating dairy cows.