کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2448340 1554013 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Metabolic characterization of rumen epithelial tissue from dairy calves fed different starter diets using 1H NMR spectroscopy
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Metabolic characterization of rumen epithelial tissue from dairy calves fed different starter diets using 1H NMR spectroscopy
چکیده انگلیسی

The metabolic profile of calf rumen epithelial tissue was for the first time characterized by 1H NMR spectroscopy of chloroform/methanol extracts. The metabolite profiles comprised a number of amino acids, creatine, taurine, short-chain fatty acids and triglycerides. The effects of two dietary interventions; i) four levels of milk allowance with a concomitant different uptake of starter concentrate, and ii) two diets with varying content of starch and fibre in the concentrate, were elucidated. Partial least square regression analysis revealed that the intensity of NMR signals assigned to leucine, isoleucine and valine (0.90  ppm), propionate (1.06 and 2.18  ppm), lactate (1.32  ppm), butyrate (1.56  ppm), acetate (1.93  ppm), glutamine/glutamate (2.18, 2.35 and 3.75  ppm), creatine (3.04 and 3.94  ppm) and glycine (3.55  ppm) decreased with increasing milk allowance. In addition, the analysis revealed that the main difference between the two diets was the content of propionate in the epithelia tissue extracts. Previous morphological analyses of the same rumen epithelia were not able to detect any significant effects of either milk allowance or dietary starch content. Accordingly, the present study demonstrated that 1H NMR spectroscopy applied on extracts is a useful tool for metabolite profiling of epithelial tissue and for following the development of epithelia tissue in young calves, and that the technique may be more sensitive to dietary effects than morphological studies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Livestock Science - Volume 120, Issues 1–2, January 2009, Pages 127–134
نویسندگان
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