کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2448800 | 1554026 | 2007 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Effect of direct-fed microbial and antibiotic supplementation on gastrointestinal microflora, mucin histochemical characterization, and immune populations of weanling pigs Effect of direct-fed microbial and antibiotic supplementation on gastrointestinal microflora, mucin histochemical characterization, and immune populations of weanling pigs](/preview/png/2448800.png)
Direct-fed microbials (DFM) have the ability to alter gastrointestinal microflora, morphology and immunity. Denaturing gradient gel electrophoresis was used to analyze the effect of Lactobacillus brevis, Bacillus, and antibiotic supplementation on the gastrointestinal microbiota. Treatments were arranged in a 2 × 3 factorial arrangement with two levels of L. brevis supplementation and three diets (control, Bacillus, and antibiotic) fed during the nursery period. Microbial diversity of the gastrointestinal microbiota increased in response to any of the treatments containing L. brevis, Bacillus, or antibiotic compared to unsupplemented pigs. Pigs provided L. brevis and Bacillus in combination exhibited more bands of high G/C content than pigs provided only the antibiotic treatment. Supplementation with L. brevis resulted in a unique band that was identified as unculturable, low G/C content, gram positive bacteria. Furthermore, L. brevis increased pig body weight at the end of the nursery by 1.6 kg compared to pigs not provided L. brevis. L. brevis increased the number of acidic goblet cells present on villi within the duodenum and jejunum, and decreased the number of sulfated goblet cells, whereas Bacillus and antibiotic supplementation decreased the number of sulfated goblet cells on villi within the duodenum. Immunohistochemical evaluation revealed that L. brevis supplementation lowered the number of CD2+ cells, antigen presenting cells (MHCII+), and CD4+ T cells within jejunal villi. These data illustrate the potential for DFM to improve pig growth rate after weaning, as well as elicit alterations in the gut microbial community, mucin-producing goblet cells, and immune cells.
Journal: Livestock Science - Volume 108, Issues 1–3, 1 May 2007, Pages 249–253