کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2448935 1109565 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection of intestinal parasites in pig slurry: A preliminary study from five farms in Spain
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Detection of intestinal parasites in pig slurry: A preliminary study from five farms in Spain
چکیده انگلیسی

The aim of this study was to investigate the presence of intestinal parasites in pig slurries from several piggeries in Alicante (Spain). Pig slurries were collected in five highly-intensive pig farms (A–E), being sampled in each farm from the pits depending on the production cycle (gestating sows, farrowing sows, weaners, finishers). Samples were concentrated either through zinc sulphate flotation or by formalin–ethyl acetate sedimentation methods. Parasitological examination was performed by optical microscopy. Detection of Cryptosporidium sp. was performed using conventional acid-fast stain and by DNA extraction and PCR amplification. Cryptosporidium genus-specific primers (CPBDIAGF and CPBDIAGR) were used to amplify the Cryptosporidium SSU-rRNA variable region. Intestinal parasites were found in all farms studied. Several protozoa (Ballantidium coli, Entamoeba coli and Cryptosposidium sp.) and helminths (Ascaris suum, Trichuris suis, Fasciola hepatica, Strongylida and nematode larvae) were identified. Parasite viability studies are needed in order to assess the potential risk for animal and human health.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Livestock Science - Volume 102, Issue 3, July 2006, Pages 237–242
نویسندگان
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