کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2450265 1109643 2012 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of meat species by PCR-RFLP of the mitochondrial COI gene
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Identification of meat species by PCR-RFLP of the mitochondrial COI gene
چکیده انگلیسی

Meat authenticity verification is pertinent for economical, religious or public health concerns. The present study investigates the use of PCR-RFLP of a part of the mitochondrial cytochrome c oxidase subunit 1 (COI) gene for identification of species origin of raw meat samples of cow, chicken, turkey, sheep, pig, buffalo, camel and donkey. PCR yielded a 710-bp fragment in all species. The amplicons were digested with seven restriction endonucleases (Hind II, Ava II, Rsa I, Taq I, Hpa II, Tru 1I and Xba I) that were selected based on the preliminary in silico analysis. Different levels of polymorphism were detected among samples. The level of COI variation revealed using only Hpa II was sufficient to generate easily analyzable species-specific restriction profiles that could distinguish unambiguously all targeted species. Compared to previously published reports for the determination of meat origin at the molecular level, the approach developed here is much cheaper and faster for routine identification of meats in food control laboratories.


► PCR-RFLP was applied on COI gene for identification of eight raw meat species.
► PCR yielded a 710-bp fragment in all species.
► The amplicons were digested with seven restriction endonucleases.
► Restriction with Hpa II was sufficient to discriminate all targeted species.
► The approach applied here is much cheaper and faster than previous methods.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Meat Science - Volume 90, Issue 2, February 2012, Pages 490–493
نویسندگان
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