کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2458541 1554426 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Allelic variations in exon 2 of caprine MHC class II DRB3 gene in Chinese indigenous goats
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Allelic variations in exon 2 of caprine MHC class II DRB3 gene in Chinese indigenous goats
چکیده انگلیسی

Allelic variations in the second exon of caprine leukocyte antigen-DRB3 gene (CLA-DRB3*02) were investigated. A total of 459 animals from 12 Chinese indigenous goat populations were genotyped. Six alleles and 18 restriction digestion profiles were distinguished by digestion of PCR amplification product of CLA-DRB3*02 with HaeIII. The results suggested that there were significant difference (P < 0.000) in both allele frequencies and genotype frequencies of exon 2 restriction fragment length polymorphisms (RFLPs) among the breeds. The new allele CLA-DRB3*0206 was identified only in three goat populations from the three-gorge reservoir, and the breed-specific allele CLA-DRB3*0205 was merely detected in the three populations of Tibetan goat. The results also indicated that the potentially different ecological factors, such as climate, disease, topography, pasture conditions and pathogens might be account for the significantly different distribution of alleles and genotypes among the goat populations. The nucleotide and predicted amino acid sequence alignment of CLA-DRB3*02 alleles provided evidence for considerable variation among the six alleles. Phylogenetic analysis showed that the six alleles of CLA-DRB3*02 were closely related. They clustered together and were separated from the corresponding sequences of sheep and cattle, which indicated the trans-species evolution model of CLA-DRB3*02 was not present in the Chinese goat populations studied.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Small Ruminant Research - Volume 66, Issues 1–3, November 2006, Pages 236–243
نویسندگان
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