کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2462815 | 1555089 | 2009 | 6 صفحه PDF | دانلود رایگان |
The effect of lidocaine on in vitro migration and adhesion of equine neutrophils was evaluated. Neutrophils were isolated from equine whole blood using a Percoll-gradient centrifugation protocol. Purified neutrophils were incubated with lidocaine at concentrations from 0.1 to 1000 μg/ml for 30 min at 37 °C, after calcein loading. Neutrophil integrin-mediated adhesion in response to stimulation with 100 nM LTB4, 100 nM PAF, or 100 ng/ml IL-8, or integrin-mediated migration in response to stimulation with 100 nM LTB4, 150 nM PAF, or 100 ng/ml IL-8 was assessed. Statistical significance was set at P < 0.05. Neutrophil adhesion was significantly increased in response to all three stimulants. IL-8-stimulated adhesion was significantly increased when neutrophils were incubated with 1 mg/ml lidocaine, compared to lower lidocaine concentrations. LTB4-stimulated adhesion was significantly increased when neutrophils were incubated with 1 mg/ml lidocaine compared to that at 5 μg/ml lidocaine. Migration was significantly increased in response to IL-8. IL-8 and LTB4 stimulated migration was significantly increased when neutrophils were incubated with 1 mg/ml lidocaine, compared to lower lidocaine concentrations. In conclusion, lidocaine did not inhibit neutrophil migration or adhesion in vitro at therapeutic concentrations, and increased migration and adhesion at higher concentrations.
Journal: Veterinary Immunology and Immunopathology - Volume 129, Issues 1–2, 15 May 2009, Pages 137–142