کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2462945 | 1555105 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular cloning and expression analysis of pig CD81
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کلمات کلیدی
LPSConcanavalin ALELPHAPhytohaemagglutininCD81Tetraspanin - tetraspaninCon A - با ACho - برایChinese Hamster Ovary - تخمدان هامستر چینیrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدPig - خوکintracellular domain - دامنه درون سلولیSel - سلولtransmembrane - فرابنفشB cell - لنفوسیت بیlipopolysaccharide - لیپوپلی ساکاریدRace - مسابقهHepatitis C virus - هپاتیت سیHCV - هپاتیت سی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
CD81, also known as TAPA-1 (target of antiproliferative antibody 1), is a member of the tetraspanin family of proteins and a component of the B cell co-receptor complex. Several studies have shown that CD81 plays significant roles in a variety of immune responses, including activation of B cells and T cells. In this study, we cloned pig Cd81 cDNA using RT-PCR coupled with rapid amplification of cDNA ends (RACE)-PCR and determined the complete cDNA sequence of pig Cd81. Pig Cd81 cDNA contains an open reading frame (711Â bp) encoding 236 amino acids. The identity of pig CD81 with those of human, cattle, rat, and mouse are 90.30%, 92.26%, 86.22%, and 86.22%, respectively. Alignment of the CD81 amino acid sequence with those of mammalian species showed that the large extracellular loop (LEL) is the most divergent, whereas other domains are largely conserved. Pig Cd81 mRNA was detected by RT-PCR in a broad range of tissues, including lymphoid tissues as well as nonlymphoid tissues, indicated variety of cellular functions of CD81 in most pig tissues. Flow cytometry analyses demonstrated that human CD81 antibody recognizes a pig CD81 on the cell surface. Further, immunohistochemistry analysis using human CD81 antibody on pig spleen was revealed that CD81 expression is widely diffused in spleen tissue. Future study will be focused on defining the functional role of CD81 during the course of pig infectious diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 120, Issues 3â4, 15 December 2007, Pages 254-259
Journal: Veterinary Immunology and Immunopathology - Volume 120, Issues 3â4, 15 December 2007, Pages 254-259
نویسندگان
Kyu-Won Cho, Sang Joon Kim, Chung-Gyu Park, Jongsun Park, Jae Youl Cho, Hyung-Sik Kang, Taehoon Chun,