کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2462998 | 1555095 | 2008 | 7 صفحه PDF | دانلود رایگان |
We previously demonstrated that intradermal (ID) delivery of plasmid DNA encoding the porcine granulocyte–macrophage colony-stimulating factor (GM-CSF) 7 days before DNA vaccination enhances both cellular and humoral responses in pigs. In the present work, we studied the effect of the GM-CSF gene on antigen-presenting cells (APC) in pigs. We demonstrated that ID delivery of this gene significantly increased the number of epidermal CD1+ cells (Langerhans’ cells, skin dendritic cells) at the injection site at day 7. This was accompanied by an enhanced percentage of APC at the immune induction site following DNA vaccination, whereas a positive effect on APC maturation could not be demonstrated. Taken together, our data suggest that both DC recruitment to the immunization site and expansion of APC in the draining LN following DNA vaccination might contribute to the immune enhancing effect of plasmid encoded GM-CSF in pigs.
Journal: Veterinary Immunology and Immunopathology - Volume 125, Issues 3–4, 15 October 2008, Pages 354–360