کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2463251 | 1555117 | 2006 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A rodent model for allergic dermatitis induced by flea antigens
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کلمات کلیدی
FACSHprtTMBPMSPBSTRT-PCRMTS3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium - 3- (4،5-dimethylthiazol-2-yl) -5- (3-carboxymethoxyphenyl) -2- (4-sulfophenyl) -2H-tetrazoliumcomplete Freund's adjuvant - adjuvant دوست کاملBSA - BSAReverse transcriptase PCR - PCR معکوس رونویسیbovine serum albumin - آلبومین سرم گاوtetramethylbenzidine - تترامتیل بنزیدینHypersensitivity - حساسیت بیش از حدstimulation index - شاخص تحریکfluorescence activated cell sorter - فلورسانس سلول فعال شده سلولphenazine methosulfate - فنزین متوسولفاتhypoxanthine phosphoribosyl transferase - هیپوکسانتین فسفریبوسیل ترانسفرازoptical density - چگالی نوری
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
There have been very few reports of experimentally induced animal models of allergic dermatitis, an immunologic disorder. This report describes the induction of histopathology confirmed allergic dermatitis in C57BL/6 mice along with the consistent clinical sign of alopecia following the administration of flea antigens emulsified in complete Freund's adjuvant (CFA). By comparing different strains of mice, routes of injection, types of adjuvants and different dosages of flea antigens, C57BL/6 mice were found to be most susceptible to flea antigens administered intramuscularly (i.m.) and subsequently developed dermatologic excoriations and local alopecia. The level of specific IgE reactive to flea antigens in C57BL/6 mice after the onset of clinical signs was significantly higher than such levels in mice without clinical signs, suggesting that flea antigen-specific IgE level can be correlated to the severity of allergic hyper-reaction. CD4+ T lymphocytes and IL-4 rather than IL-10, or IFN-γ were found to be the predominant cytokines associated with the clinical onset of allergic symptoms in C57BL/6 mice. Further, histopathologic analysis indicated that not only mast cells had infiltrated into the area of the skin lesion, but the damage was found to be at a stage where mast cells were degranulating causing considerable exacerbation of the local injury. In conclusion, this murine allergic dermatitis model induced by flea antigens may provide a useful means to evaluate vaccines or immunodulatory drugs; thus providing researchers with a tool to study allergy-related disorders and other parameters needed in the area of allergic investigations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 114, Issues 3â4, 15 December 2006, Pages 285-296
Journal: Veterinary Immunology and Immunopathology - Volume 114, Issues 3â4, 15 December 2006, Pages 285-296
نویسندگان
Lin Zhao, Huali Jin, Ruiping She, Yanxin Hu, Chong Xiao, Yang Yu, Junpeng Wang, Fengqiang Sun, Terry Ng, Steve Chu, Bin Wang,