کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2463382 | 1555120 | 2006 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The N-acetyl-d-glucosamine specific adhesin from Mannheimia haemolytica activates bovine neutrophils oxidative burst The N-acetyl-d-glucosamine specific adhesin from Mannheimia haemolytica activates bovine neutrophils oxidative burst](/preview/png/2463382.png)
In this work we identified specific bovine leukocytes that were bound by the Mannheimia haemolytica adhesin molecule (MhA) and the biological effect on the leukocytes. Histochemical staining and flow cytometry showed that MhA bind neutrophils (90%) and monocytes (5%). MhA induced an oxidative response in purified neutrophils; this effect was 1.5-fold higher than the effect observed with control cells activated with Zymosan. Cellular binding by MhA was inhibited with GlcNAc and its oligomers, as well as by glycoproteins containing tri- and tetra-antennary N-glycosydically linked glycans. MhA-induced oxidative burst was significantly inhibited by GlcNAc, iodoacetamide, superoxide dismutase, and piroxicam (p < 0.05). Our findings suggest that among bovine leukocytes, neutrophils are the main target for MhA, inducing production of oxidative radicals by non-opsonic mechanism that seem to play an important role in tissue damage during mannheimiosis.
Journal: Veterinary Immunology and Immunopathology - Volume 113, Issues 1–2, 15 September 2006, Pages 148–156