کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2463551 1555125 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Tumor necrosis factor-alpha enhances Haemophilus somnus lipooligosaccharide-induced apoptosis of bovine endothelial cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Tumor necrosis factor-alpha enhances Haemophilus somnus lipooligosaccharide-induced apoptosis of bovine endothelial cells
چکیده انگلیسی

Haemophilus somnus lipooligosaccharide (LOS)-induced apoptosis of bovine pulmonary artery endothelial cells has been shown previously to be dependent on capsase-8 activation. Activation of caspase-8 can occur via a death receptor-dependent mechanism (e.g., TNF-α binding to TNF-α receptor 1 (TNF-R1)). In this study, we tested the hypothesis that TNF-α can enhance LOS-induced apoptosis of bovine endothelial cells. Addition of exogenous recombinant human TNF-α alone failed to cause apoptosis, or enhance LOS-induced apoptosis, of bovine endothelial cells. However, blocking de novo protein synthesis by addition of cycloheximide significantly enhanced apoptosis of bovine endothelial cells by TNF-α, LOS or TNF-α and LOS in combination. Conversely, addition of soluble recombinant human (sTNF-R1) diminished LOS-induced apoptosis. Overall, these data suggest that LOS-mediated apoptosis may be due, in part, to activation of a TNR-R1-dependent death pathway.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 110, Issues 3–4, 15 April 2006, Pages 303–309
نویسندگان
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