کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2463627 1555127 2006 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of serum immunoglobulin M of grouper and cDNA cloning of its heavy chain
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Characterization of serum immunoglobulin M of grouper and cDNA cloning of its heavy chain
چکیده انگلیسی

Immunoglobulin M (IgM) from the whole serum of grouper fish, Epinephelus coioides was purified by affinity chromatography using protein A-Sepharose column. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions revealed that the relative molecular masses (Mr) of the equimolar heavy and light chains of IgM were 78,000 and 27,000, respectively. The cDNAs encoding IgM heavy chain comprising its variable (VH) and constant (CH) regions have been cloned and sequenced from a grouper kidney cDNA library by antibody screening method. Five VH (130–142 amino acids) and four CH (450–454 amino acids) families were identified. The variable and constant regions were conserved with their putative domains. All the four constant region domains (CH1–CH2–CH3–CH4) contained each three conserved cysteine residues, which are considered to form the inter- and intra-chain disulfide bridges. There were three carbohydrate acceptor sites in the constant region. In general, the pattern of IgM gene organization seems to resemble that of other teleosts. Moreover, the CH genes in grouper IgM occur as multifamily as reported in Atlantic salmon and common carp.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 109, Issues 3–4, 15 February 2006, Pages 255–265
نویسندگان
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