Isolation and molecular characterization of bovine enteroviruses in Egypt

• Bovine enterovirus (BEV) was isolated from diarrheic calves in Egypt.
• Sequencing revealed of BEV/Egypt/2014//KM667941 to have 7417 nucleotides (nt).
• The organization of nt was typical of the BEV genome including 822 nt in 5′NTR, 6498 nt in ORF, and 97 nt in 3′NTR.
• Phylogenetic analysis revealed clustering of BEV/Egypt/2014/KM667941 with BEV-F.
• A specific RT-PCR for BEV was developed to detect this pathogen in cattle.

Enteroviruses belong to the Picornaviridae family and infect a wide range of mammals including cattle. Bovine enterovirus (BEV) has recently been reclassified into E and F serotypes. BEV was first isolated in Egypt in 1966 although it has been known in other countries since the 1950s. In this study, BEV-F2 was isolated from calves with severe diarrhea and the isolated viruses were subjected to molecular characterization. Illumina sequencing of one of the isolates revealed the presence of a complete BEV-F genome sequence. The phylogenetic analysis revealed nucleotide substitutions along the genome in comparison with other known strains of BEV-F (HQ663846, AY508697 and DQ092795). Two primer sets were designed from the 3D and 5′NTR regions and used for the examination of the remaining isolates, which were confirmed to be of the BEV-F2 serotype. The availability of the complete genome sequence of this virus adds to the sequence database of the members of Picornaviridae and should be useful in future molecular studies of BEV.