کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2464000 1111769 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Clinical, virological, immunological and pathological evaluation of four porcine circovirus type 2 vaccines
ترجمه فارسی عنوان
ارزیابی بالینی، ویروسی، ایمونولوژیک و آسیب شناسی واکسن های نوع 2 دیابتی ویروسی خوک
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی

The objective of this study was to rigorously compare the efficacy of four porcine circovirus type 2 (PCV2) vaccines of varying antigen type and dose under experimental conditions based on well-defined clinical (average daily weight gain [ADWG]), virological (evidence of viraemia), immunological (presence of PCV2-specific neutralising antibodies [NA], interferon-γ-secreting cells [IFN-γ-SCs], and CD3+ and CD4+ T cell subsets), and pathological (lymphoid lesion and PCV2 antigen score) criteria. A total of 60, 3-week old piglets were assigned to six groups of 10/group and were vaccinated either with 1/4 commercially available one-dose vaccines or were not vaccinated. At 7 weeks of age, vaccinated and control animals were inoculated intranasally with 2 mL of PCV2b. All pigs were euthanased and subjected to post-mortem examination at 25 weeks of age.From 9 to 16 weeks of age, the ADWG of vaccinated animals was significantly higher than that of non-vaccinates. Significant (P < 0.05) differences were observed between vaccinated and positive control groups in the quantity of log-transformed PCV2b DNA in the blood and nasal swabs, log-transformed NA titres, and PCV2-specific IFN-γ-SCs at 0, 7, 14, 21, and 42 days post challenge (dpc). The proportion of CD4+ cells at 7 and 14 dpc was also significantly different between vaccinated and control pigs (P < 0.05). The histopathological lesions and PCV2-antigen scores in the lymph nodes were significantly lower (P < 0.05) in vaccinated animals. All four vaccines were found to be highly efficacious in controlling experimental PCV2 challenge based on this range of criteria.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Veterinary Journal - Volume 200, Issue 1, April 2014, Pages 65–70
نویسندگان
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