کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2465672 | 1111836 | 2008 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The effect of chondrocyte cryopreservation on cartilage engineering The effect of chondrocyte cryopreservation on cartilage engineering](/preview/png/2465672.png)
Chondrocytes were collected from the stifle joints of four pigs to study the effect of cryopreservation on the chondrogenic potential of chondrocytes. Half of the cells were cryopreserved for 3 months. Polyglycolic acid scaffolds were cultured with fresh or cryopreserved chondrocytes for 4 weeks. Cell morphology and the quality of engineered tissue were evaluated by scanning electron microscopy, histopathology and biochemical methods. More cells attached to scaffolds at 48 h when fresh chondrocytes were seeded. At 4 weeks, the numbers of cells, DNA and collagen II were greater in constructs engineered by fresh cells. However, the collagen II/DNA ratio did not differ between the two groups. More matrix was identified on a scanning electron microscope and by histopathology in the fresh group. Cartilage engineered with cryopreserved chondrocytes may contain less matrix and fewer cells. These findings most likely resulted from a lack of cell attachment on the matrix secondary to cryopreservation. Future studies are needed to further evaluate the mechanism by which cryopreservation may affect chondrocyte attachment.
Journal: The Veterinary Journal - Volume 178, Issue 2, November 2008, Pages 244–250