کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2465784 1111839 2007 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular profiling and comparison of field transmissible spongiform encephalopathy cases diagnosed in Catalunya
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Molecular profiling and comparison of field transmissible spongiform encephalopathy cases diagnosed in Catalunya
چکیده انگلیسی

Molecular profiling of the proteinase K resistant prion protein (PrPres) is a technique that has been applied to the characterisation of transmissible spongiform encephalopathy (TSE) strains. An interesting example of the application of this technique is the ability to differentiate, at the experimental level, between bovine spongiform encephalopathy (BSE) and scrapie infection in sheep, and to distinguish between classical and atypical BSE and scrapie cases. Twenty-six BSE cases and two scrapie cases from an active TSE surveillance program and diagnosed at the PRIOCAT, TSE Reference Laboratory (Centre de Recerca en Sanitat Animal, Universitat Autònoma de Barcelona, Catalunya, Spain) were examined by Western blotting. Molecular profiling was achieved by comparing the glycosylation profile, deglycosylated PrP molecular weight and 6H4/P4 monoclonal antibody binding ratio. The results obtained during the characterisation of these field cases indicated an absence of atypical BSE cases in Catalunya.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Veterinary Journal - Volume 174, Issue 1, July 2007, Pages 196–199
نویسندگان
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