Genetic vaccination by gene electro-transfer in non-human primates

Muscle gene electro-transfer (GET) of plasmid DNA is a promising approach for gene therapy and genetic vaccination. Several protocols have been described which give good levels of gene transduction in small animals. However, to progress towards human applications, efficacy must be demonstrated in non-human primates. Here, we extensively explore several electrical and injection parameters in Rhesus monkeys and define a series of conditions for efficient vaccination.