Preparation of CyA-loaded solid lipid nanoparticles and application on ocular preparations

Cyclosporine A as a model drug, drug loaded solid lipid nanoparticles (SLNs) were prepared with glyceryl monostearate and Compritol ATO 888 using the ultrasonication method. The characteristics of SLN were assessed by particle size analysis, zeta potential measurement, entrapment efficiency, physical stability andpre-ocular retention study. It demonstrated that the particle size and stability of the SLNs were greatly influenced by the formulation including the composition of lipid matrix, surfactant, charge modifier and the drug amount. The CyA-loaded SLNs produced by optimized formulation showed a good particle size distribution, the average particle size, zeta potential and entrapment efficiency was 122 nm, + 23 mv and 93%, respectively, as well as good physical stability. The ocular retention study indicated that the SLNs coated with plus charge modifier enhanced ocular retention time on the cornea surface compared with SLNs without surface modifier.