کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2495563 1116069 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Estimation of sertraline by chromatographic (HPLC-UV273nm) technique under hydrolytic stress conditions
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی پزشکی مولکولی
پیش نمایش صفحه اول مقاله
Estimation of sertraline by chromatographic (HPLC-UV273nm) technique under hydrolytic stress conditions
چکیده انگلیسی
Purpose: In this paper, simple, specific and accurate RP-HPLC method was developed in order to study decomposition of sertraline (SRT) under the hydrolytic stress conditions (acid, neutral, alkaline and oxidative). Materials and Methods: The best separation of SRT and its degradation products were achieved on reverse phase LiChoCART with Purospher (RP-18e) column. The mobile phase was composed of methanol/water (75:25, v/v). The detection wavelength was 273 nm. The method was validated and response was found to be linear in the drug concentration range of 10-200 μg ml-1 with correlation coefficient of 0.998. Results: The RSD values for intra- and inter-day precision were < 0.65 and < 0.72%, respectively. Employing RP-HPTC method, degradation products were detected in the exposed samples. Conclusion: It was found that the susceptibility of SRT to hydrolytic decomposition increased in following manner: Neutral condition < alkaline condition < acid condition < oxidative condition.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pharmaceutical Methods - Volume 3, Issue 2, July–December 2012, Pages 62-67
نویسندگان
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