کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2496005 | 1116099 | 2011 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
HPTLC Fingerprinting Profile of Marker Compound (Berberine) in Roots of Berberis aristata DC
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
پزشکی مولکولی
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چکیده انگلیسی
A sensitive and reliable densitometric High Performance Thin Layer Chromatography method has been developed for the quantification of berberine, an alkaloid present in roots of Berberis aristata. Chromatographic analysis was performed using methanol extract of roots of Berberis aristata on silica gel 60F254 GLP (E. Merck) plates using the solvent system, n-propanol: formic acid: water (9:01:0.9). Detection and quantification of berberine was done by densitometric scanning at 364 nm. The results of linearity range and correlation coefficient show that there was a good correlation between peak area and corresponding concentration of berberine. The proposed HPTLC method provided a good resolution of berberine from other constituents present in methanol extract of roots of B. aristata and can be used for the quantification of berberine.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pharmacognosy Journal - Volume 3, Issue 19, January 2011, Pages 41-44
Journal: Pharmacognosy Journal - Volume 3, Issue 19, January 2011, Pages 41-44
نویسندگان
Rashmi Rashmi, Jagdish Pant, A. Rajasekaran,